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KMID : 0881720180330030207
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 3 p.207 ~ p.213
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Genotoxicity Study of Litsea japonica Fruit Flesh Extract
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Yun Ji-Hyun
Park In-Jae
Park Sung-Hwan
Choi Goo-Hee
Kim Hyun-Jung
Cho Ju-Hyun
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Abstract
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This study aimed to evaluate the genotoxicity of Litsea japonica fruit-hexane extract (LJF-HE). In order to examine the genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, and a micronucleus induction (MN) test according to the OECD and the Korea Ministry of Food and Drug Safety (MFDS) toxicity test guidelines. In the bacterial reverse mutation assay, no significant increase in revertant colonies, nor bacterial toxicity, was observed in the LJF-HE treatment group, regardless of the absence or presence of metabolic activation by the S9 mixture. However, in the positive control group, revertant colony counts were shown to be more than twice that of the negative control group. The chromosome aberration test showed that the repetition rate of abnormal chromosome aberration was less than 5%, regardless of the treatment time, and with or without the S9 mixture. No significant change was observed when (p<0.05) compared with the negative control group. The micronucleated polychromatic erythrocytes (MNPCE) repetition rate of the polychromatic erythrocytes (PCE) showed no significant changes when compared with the negative control group (p<0.05). The PCE portion of total erythrocytes also showed no significant changes (p<0.05). These results showed that LJF-HE had no significant genotoxic effects.
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KEYWORD
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Litsea japonica, Genotoxicity, Reverse mutation, Micronucleus test
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